Polyclonal antibody to Phospho-CBL-Y700
WB, IHC, IF
CBL antibody; C-CBL antibody; CBL2 antibody; FRA11B antibody; NSLL antibody; RNF55 antibody; Cbl proto-oncogene antibody
Antigen: A phospho specific peptide corresponding to residues surrounding Y700 of human CBL
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:20 - 1:100
Western blot - Phospho-CBL-Y700 pAb
Western blot analysis of extracts of HT-29 cells, using Phospho-CBL-Y700 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-CBL-Y700 pAb
Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-CBL-Y700 antibody .
Immunofluorescence - Phospho-CBL-Y700 pAb
Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-CBL-Y700 antibody .
Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).
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