Polyclonal antibody to Phospho-BCR-Y177
BCR antibody; ALL antibody; BCR1 antibody; CML antibody; D22S11 antibody; D22S662 antibody; PHL antibody; breakpoint cluster region protein antibody
Antigen: A synthetic phosphorylated peptide around Y177 of human BCR (NP_004318.3).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-BCR-Y177 pAb
Western blot analysis of extracts of K562 cells, using Phospho-BCR-Y177 antibody at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 90s.
GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"