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  • Phospho-BCR-Y177 pAb

Phospho-BCR-Y177 pAb

Cat.#: 166492

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Product Information

  • Product Name
    Phospho-BCR-Y177 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-BCR-Y177
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse
  • Alternative names
    BCR antibody; ALL antibody; BCR1 antibody; CML antibody; D22S11 antibody; D22S662 antibody; PHL antibody; breakpoint cluster region protein antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around Y177 of human BCR (NP_004318.3).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - Phospho-BCR-Y177 pAb

    Western blot - Phospho-BCR-Y177 pAb

    Western blot analysis of extracts of K562 cells, using Phospho-BCR-Y177 antibody at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"