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Product Name
Phospho-AMPKβ1-S108 pAb
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Description
Polyclonal antibody to Phospho-AMPKβ1-S108
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Tested applications
WB
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Species reactivity
Human
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Alternative names
PRKAB1 antibody; AMPK antibody; HAMPKb antibody; 5'-AMP-activated protein kinase subunit beta-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphorylated peptide around S108 of human AMPKβ1 (NP_006244.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - Phospho-AMPKu03b21-S108 pAb
Western blot analysis of extracts of 293 cells, using Phospho-AMPKu03b21-S108 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
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Background
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"