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Gene name
penta-EF hand domain containing 1
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Organism
Mouse
- Entrez Gene ID
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Gene Symbol
Pef1
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Gene Type
protein coding
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Also Known as
Peflin; 2600002E23Rik
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Encoded Protein Alias
PEF protein with a long N-terminal hydrophobic domain; penta-EF hand domain-containing protein 1
Accession NO.: NM_026441
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Product Name
Pef1(NM_026441) Mouse cDNA/ORF Clone
- Accession NO.
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Transcript Name
Mus musculus penta-EF hand domain containing 1 (Pef1), mRNA.
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Synonyms
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ORF length
828
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siRNA
ACUGGUCCUCAUUCAAUGAtt
GAACCUCUUUCAGCAGUAUtt
GGAGGAACCUCUUUCAGCAtt
GCCUCAUGAUGAUAAACAUtt
- DataSheet:
- DataSheet:
- FAQs:
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Vector Name
pEXP-Entry
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primers
T7: TAATACGACTCACTATAGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4919
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
Myc,FLAG
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Description
ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
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Vector Name
pUC19
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primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2686
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
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