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Gene name
paired box 6
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Organism
Human
- Entrez Gene ID
-
Gene Symbol
PAX6
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Gene Type
protein coding
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Also Known as
AN; AN2; FVH1; MGDA; WAGR; D11S812E
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Encoded Protein Alias
paired box protein Pax-6; aniridia type II protein; oculorhombin; paired box homeotic gene-6
Accession NO.: NM_001604
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Product Name
PAX6(NM_001604) Human cDNA/ORF Clones
- Accession NO.
-
Transcript Name
Homo sapiens paired box 6 (PAX6), transcript variant 4, mRNA.
- Synonyms
-
ORF length
1311
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Annotation
This variant (4) differs in the 5' UTR and includes an alternate in-frame exon in the 5' coding region, compared to variant 1. It initiates from the A (P0) promoter. The encoded isoform (b, also known as 5a) is longer than isoform a. Variants 2, 4, 5 and 8 encode the same isoform (b).
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siRNA
GGCAAUCGGUGGUAGUAAAtt
CCAACUCCAUCAGUUCCAAtt
CCAAGCGUGUCAUCAAUAAtt
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Accession NO.: NM_000280
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Product Name
PAX6(NM_000280) Human cDNA/ORF Clones
- Accession NO.
-
Transcript Name
Homo sapiens paired box 6 (PAX6), transcript variant 1, mRNA.
- Synonyms
-
ORF length
1269
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Annotation
This variant (1) initiates from the B (P1) promoter and encodes isoform a. Variants 1, 3, 6 and 7 encode the same isoform (a).
-
siRNA
GGCAAUCGGUGGUAGUAAAtt
CCAACUCCAUCAGUUCCAAtt
CCAAGCGUGUCAUCAAUAAtt
- DataSheet:
- FAQs:
Accession NO.: NM_001310159
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Product Name
PAX6(NM_001310159) Human cDNA/ORF Clones
- Accession NO.
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Transcript Name
Homo sapiens paired box 6 (PAX6), transcript variant 9, mRNA.
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Synonyms
-
ORF length
1206
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Annotation
This variant (9) has a shorter 5' UTR and lacks multiple 3' coding exons compared to variant 1. Its transcription extends past a splice site used in variant 1, resulting in a distinct 3' coding region and 3' UTR. The encoded isoform (c, also known as S) has a distinct, shorter C-terminus than isoform a.
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siRNA
GGCAAUCGGUGGUAGUAAAtt
CCAACUCCAUCAGUUCCAAtt
CCAAGCGUGUCAUCAAUAAtt
- DataSheet:
- FAQs:
Accession NO.: NM_001310160
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Product Name
PAX6(NM_001310160) Human cDNA/ORF Clones
- Accession NO.
-
Transcript Name
Homo sapiens paired box 6 (PAX6), transcript variant 10, mRNA.
- Synonyms
-
ORF length
861
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Annotation
This variant (10) initiates from the P alpha promoter and contains multiple differences in the 5' region, compared to variant 1. The encoded isoform (d) has a shorter N-terminus and lacks the paired domain, compared to isoform a. Variants 10 and 11 encode the same isoform (d).
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siRNA
GGCAAUCGGUGGUAGUAAAtt
CCAACUCCAUCAGUUCCAAtt
- DataSheet:
- FAQs:
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Vector Name
pCMV-SPORT6
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4396
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
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Selection Marker
-
-
Protein Tag
None
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Description
Vector for cloning and transient mammalian cell expression of cDNAs.
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Vector Name
pEXP-C-EGFP
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6703
-
Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Hygromycin
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Protein Tag
GFPSpark
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-FLAG
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6013
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
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Protein Tag
FLAG
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-mOrange2
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6662
-
Vector Type
Mammalian Expression Vector
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Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
OFPSpark
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-N-EGFP
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6703
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Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
GFPSpark
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-N-FLAG
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6013
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
FLAG
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-untagged
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6223
-
Vector Type
Mammalian Expression Vector
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Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Hygromycin
-
Protein Tag
None
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pGEM-T
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3000
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
-
Protein Tag
None
-
Description
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
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Vector Name
pOTENT-1
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primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
FLAG, 6 His
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Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Vector Name
pcDNA3.1-3xFlag
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5508
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Neomycin
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Protein Tag
Flag
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Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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