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Product Name
NUP62 antibody
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Description
NUP62 Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, NIH/3T3 cells. Positive IP detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 62kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
62 kDa nucleoporin antibody; DKFZp547L134 antibody; FLJ20822 antibody; FLJ43869 antibody; IBSN antibody; Nuclear pore glycoprotein p62 antibody; nucleoporin 62kDa antibody; Nucleoporin Nup62 antibody; NUP62 antibody; p62 antibody; SNDI antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NUP62 recombinant protein (Accession Number: NM_153719). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113381(NUP62 antibody) at dilution of 1:1500
IP Result of anti-NUP62 (IP:Catalog No:113381, 4ug; Detection:Catalog No:113381 1:1000) with NIH/3T3 cells lysate 1200ug.
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Background
NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)
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References
- Béaslas O, Vihervaara T, Li J, Laurila PP, Yan D, Olkkonen VM. Silencing of OSBP-related protein 8 (ORP8) modifies the macrophage transcriptome, nucleoporin p62 distribution, and migration capacity. Experimental cell research. 318(15):1933-45. 2012.
- Rose K, Ooi L, Dalle C, Robertson B, Wood IC, Gamper N. Transcriptional repression of the M channel subunit Kv7.2 in chronic nerve injury. Pain. 152(4):742-54. 2011.
- Zhou T, Li S, Zhong W. OSBP-related protein 8 (ORP8) regulates plasma and liver tissue lipid levels and interacts with the nucleoporin Nup62. PloS one. 6(6):e21078. 2011.
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