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Product Name
NUDT21 antibody
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Description
NUDT21 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human brain tissue, human cervical cancer tissue. Positive IF detected in MCF-7 cells. Positive IP detected in HeLa cells. Positive WB detected in human brain tissue, A431 cells, HEK-293 cells, HeLa cells. Observed molecular weight by Western-blot: 26kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CFIM25 antibody; CPSF 25 kDa subunit antibody; CPSF25 antibody; CPSF5 antibody; DKFZp686H1588 antibody; Nudix motif 21 antibody; NUDT21 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NUDT21 recombinant protein (Accession Number: NM_007006). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113360(NUDT21 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:113360(NUDT21 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using NUDT21 antibody Catalog No:113360 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NUDT21 (IP:Catalog No:113360, 3ug; Detection:Catalog No:113360 1:500) with HeLa cells lysate 3000ug.
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Background
Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.
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References
- Kim S, Yamamoto J, Chen Y. Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation. Genes to cells : devoted to molecular & cellular mechanisms. 15(9):1003-13. 2010.
- Masamha CP, Xia Z, Yang J. CFIm25 links alternative polyadenylation to glioblastoma tumour suppression. Nature. 510(7505):412-6. 2014.
- Misra A, Ou J, Zhu LJ, Green MR. Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors. Molecular cell. 58(5):819-31. 2015.
- Yao X, Tang Z, Fu X. The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination. The EMBO journal. 34(23):2885-902. 2015.
- Sartini BL, Wang H, Wang W, Millette CF, Kilpatrick DL. Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis. Biology of reproduction. 78(3):472-82. 2008.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"