NGEF Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
Ephexin-1 antibody; Eph-interacting exchange protein antibody; Neuronal guanine nucleotide exchange factor antibody; NGEF antibody
This NGEF antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 669-704 amino acids from the C-terminal region of human NGEF.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
All lanes : Anti-NGEF Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: PC-3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).
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