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Product Name
NGEF Antibody (C-term)
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Description
Purified Rabbit Polyclonal Antibody (Pab)
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Tested applications
WB, ELISA
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Species reactivity
Human
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Alternative names
Ephexin-1 antibody; Eph-interacting exchange protein antibody; Neuronal guanine nucleotide exchange factor antibody; NGEF antibody
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Isotype
Rabbit IgG
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Preparation
This NGEF antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 669-704 amino acids from the C-terminal region of human NGEF.
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Clonality
polyclonal
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Storage instructions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
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Applications
WB: 1:2000
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Validations
All lanes : Anti-NGEF Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: PC-3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Background
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"