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Gene name
neuroepithelial cell transforming 1
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Organism
Human
- Entrez Gene ID
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Gene Symbol
NET1
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Gene Type
protein coding
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Also Known as
NET1A; ARHGEF8
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Encoded Protein Alias
neuroepithelial cell-transforming gene 1 protein; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelioma transforming gene 1; p65 Net1 proto-oncogene protein; small GTP-binding protein regulator
Accession NO.: NM_001047160
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Product Name
NET1(NM_001047160) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
Homo sapiens neuroepithelial cell transforming 1 (NET1), transcript variant 1, mRNA.
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Synonyms
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ORF length
1791
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Annotation
This variant (1) represents the longest transcript and encodes the longer isoform (1).
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siRNA
GCCCAGAAGUUUUCUAGCAtt
GGAACGAACGGCACUCUUAtt
GAAGUCGCCUAGUCAAAUAtt
- DataSheet:
- DataSheet:
- FAQs:
Accession NO.: NM_005863
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Product Name
NET1(NM_005863) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
Homo sapiens neuroepithelial cell transforming 1 (NET1), transcript variant 2, mRNA.
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Synonyms
-
ORF length
1629
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Annotation
This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.
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siRNA
GCCCAGAAGUUUUCUAGCAtt
GGAACGAACGGCACUCUUAtt
GAAGUCGCCUAGUCAAAUAtt
- DataSheet:
- DataSheet:
- DataSheet:
- FAQs:
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Vector Name
pCMV-SPORT6
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4396
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
Vector for cloning and transient mammalian cell expression of cDNAs.
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Vector Name
pGEM-T
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3000
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
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Vector Name
pOTENT-1
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primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
FLAG, 6 His
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Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"