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Product Name
Necitumumab reference antibody
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Description
Anti-ERBB1 / EGFR / HER1 Antibody (Necitumumab)
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Tested applications
ELISA
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Species reactivity
Human ERBB1 / EGFR / HER1
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Isotype
IgG1
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Preparation
Recombinant expression and purified from CHO cells.
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Clonality
Monoclonal
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Formulation
0.1 M Pro-Ac, 20 mM Arg, pH 5.0
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Storage instructions
-80°C for 2 years under sterile conditions; -20°C for 1 year under sterile conditions; Avoid repeated freeze-thaw cycles.
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Validations
SDS-PAGE
Anti-ERBB1 / EGFR / HER1 Antibody (necitumumab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of Anti-ERBB1 / EGFR / HER1 Antibody (necitumumab) is more than 95%, determined by SEC-HPLC.
Bioactivity: ELISA
Immobilized human EGFR His at 2 ug/mL can bind Anti-ERBB1 / EGFR / HER1 Antibody (necitumumab), EC50=0.001519 ug/mL.
Bioactivity: FACS
Human EGFR CHO-K cells were stained with Anti-ERBB1 / EGFR / HER1 Antibody (necitumumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.08413 ug/mL.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"