Mouse Hmga2 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
Hmgic antibody; High mobility group protein HMGI-C antibody; High mobility group AT-hook protein 2 antibody; Hmga2 antibody; Hmgic antibody
This Mouse Hmga2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 72-105 amino acids from the C-terminal region of Mouse Hmga2.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
WB: 1:1000, IF: 1:25
All lanes : Anti-Hmga2 Antibody (C-term) at 1:1000 dilution Lane 1: F9 whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: NCCIT whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 12 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent image of C2C12cells stained with Mouse Hmga2 Antibody (C-term)(Cat#134880). 134880 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes.
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