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Gene name
kinase insert domain receptor
-
Organism
Human
- Entrez Gene ID
-
Gene Symbol
KDR
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Gene Type
protein coding
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Also Known as
FLK1; CD309; VEGFR; VEGFR2
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Encoded Protein Alias
vascular endothelial growth factor receptor 2; fetal liver kinase 1; fetal liver kinase-1; kinase insert domain receptor (a type III receptor tyrosine kinase); protein-tyrosine kinase receptor Flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor
Accession NO.: NM_002253
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Product Name
KDR(NM_002253) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens kinase insert domain receptor (KDR), mRNA.
-
Synonyms
-
ORF length
4071
-
siRNA
CAUGUUCUCUAAUAGCACAtt
CCAUCGUCAUGGAUCCAGAtt
GACGGACAGUGGUAUGGUUtt
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Vector Name
pEXP-C-EGFP
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6703
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
GFPSpark
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-FLAG
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6013
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
FLAG
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-C-HA
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6016
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
HA
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-C-His
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6019
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
His
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-C-Myc
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6019
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
Myc
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-C-mOrange2
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6662
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
OFPSpark
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-N-HA
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6016
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
HA
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-N-His
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6019
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
His
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-N-Myc
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6019
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
Myc
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-SP-N-FLAG
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6058
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
FLAG
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-untagged
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6223
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Hygromycin
-
Protein Tag
None
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pOTENT-1
-
primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Neomycin
-
Protein Tag
FLAG, 6 His
-
Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
-
Vector Name
pUC18c
-
primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2693
-
Vector Type
Cloning Vector
-
Promoter
lac
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
-
-
Protein Tag
None
-
Description
pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
-
Vector Name
pcDNA3.1-3xFlag
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5494
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Neomycin
-
Protein Tag
Flag
-
Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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