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Gene name
interleukin 2
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Organism
Rat
- Entrez Gene ID
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Gene Symbol
Il2
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Gene Type
protein coding
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Encoded Protein Alias
interleukin-2; IL-2; T-cell growth factor; TCGF
Accession NO.: NM_053836
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Product Name
Il2(NM_053836) Rat cDNA/ORF Clones
- Accession NO.
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Transcript Name
Rattus norvegicus interleukin 2 (Il2), mRNA.
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Synonyms
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ORF length
468
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siRNA
CAAUAUCAGAGUAACUGUUtt
GGAUCGAUAAUUACAAGAAtt
CCUGGAAAAUGAACUCGGAtt
- DataSheet:
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Vector Name
pUC18c
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primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2693
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
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