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Gene name
interleukin 17A
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Organism
Human
- Entrez Gene ID
-
Gene Symbol
IL17A
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Gene Type
protein coding
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Also Known as
IL17; CTLA8; IL-17; CTLA-8; IL-17A
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Encoded Protein Alias
interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
Accession NO.: NM_002190
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Product Name
IL17A(NM_002190) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
Homo sapiens interleukin 17A (IL17A), mRNA.
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Synonyms
-
ORF length
468
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siRNA
AGGUCCUCAGAUUACUACAtt
CCAUAACCGGAAUACCAAUtt
GGACUGUGAUGGUCAACCUtt
UACCAAUACCAAUCCCAAAtt
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Vector Name
pEXP-C-EGFP
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6703
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Hygromycin
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Protein Tag
GFPSpark
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-FLAG
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6013
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
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Protein Tag
FLAG
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-HA
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6016
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
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Protein Tag
HA
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-His
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6019
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
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Protein Tag
His
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-Myc
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6019
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Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
Myc
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-mOrange2
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6662
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Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
OFPSpark
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-SP-N-FLAG
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6058
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Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
FLAG
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-untagged
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6223
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Vector Type
Mammalian Expression Vector
-
Promoter
CMV
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Antibiotic Resistance
Ampicillin
-
Selection Marker
Hygromycin
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Protein Tag
None
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pGEM-T
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3000
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
-
Protein Tag
None
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Description
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
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Vector Name
pOTENT-1
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primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
FLAG, 6 His
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Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"