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Product Name
IKB alpha (IKBA) Antibody
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Description
Purified Rabbit Polyclonal Antibody (Pab)
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Tested applications
WB, ELISA
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Species reactivity
Human, Mouse, Rat
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Alternative names
IKBA antibody; MAD3 antibody; NFKBI antibody; NF-kappa-B inhibitor alpha antibody; I-kappa-B-alpha antibody; IkB-alpha antibody; IkappaBalpha antibody; Major histocompatibility complex enhancer-binding protein MAD3 antibody; NFKBIA antibody; IKBA antibody; MAD3 antibody; NFKBI antibody
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Isotype
Rabbit IgG
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Preparation
This IKB alpha (IKBA) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human IKB alpha (IKBA).
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Clonality
Polyclonal
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Formulation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage instructions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
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Applications
WB: 1:2000
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Validations
All lanes : Anti-Sumo-site. NFkB Antibody at 1:2000 dilution Lane 1: Daudi whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Background
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"