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Gene name
fumarate hydratase
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Organism
Human
- Entrez Gene ID
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Gene Symbol
FH
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Gene Type
protein coding
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Also Known as
MCL; FMRD; LRCC; HLRCC; MCUL1
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Encoded Protein Alias
fumarate hydratase, mitochondrial; fumarase
Accession NO.: NM_000143
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Product Name
FH(NM_000143) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
Homo sapiens fumarate hydratase (FH), mRNA.
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Synonyms
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ORF length
1533
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siRNA
GCAGAGCAGUUUGACGAAUtt
GGAUAGAAUAUGAUACCUUtt
CCAACGAUCAUGUUAAUAAtt
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- FAQs:
Accession NO.: XM_011544132
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Product Name
FH(XM_011544132) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
PREDICTED: Homo sapiens fumarate hydratase (FH), transcript variant X1, mRNA.
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Synonyms
-
ORF length
1305
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siRNA
GCAGAGCAGUUUGACGAAUtt
GGAUAGAAUAUGAUACCUUtt
CCAACGAUCAUGUUAAUAAtt
- DataSheet:
- FAQs:
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Vector Name
pEXP-Entry
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primers
T7: TAATACGACTCACTATAGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4919
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
Myc,FLAG
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Description
ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
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Vector Name
pOTB7
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primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
1815
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Vector Type
Cloning Vector
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Promoter
-
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Antibiotic Resistance
Chloramphenicol
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Selection Marker
-
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Protein Tag
None
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Description
Gateway® or compatible clone with M13 forward and M13 reverse primers. Chloramphenicol resistance in bacteria.
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Vector Name
pOTENT-1
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primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
FLAG, 6 His
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Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Vector Name
pUC18c
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primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2693
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
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Vector Name
pcDNA3.1-3xFlag
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5494
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
Neomycin
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Protein Tag
Flag
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Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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