Purified Mouse Monoclonal Antibody (Mab)
FC, WB, IHC-P, ELISA
DRT antibody; EPHT3 antibody; EPTH3 antibody; ERK antibody; HEK5 antibody; TYRO5 antibody; Ephrin type-B receptor 2 antibody; Developmentally-regulated Eph-related tyrosine kinase antibody; ELK-related tyrosine kinase antibody; EPH tyrosine kinase 3 antibody; EPH-like kinase 5 antibody; EK5 antibody; hEK5 antibody; Renal carcinoma antigen NY-REN-47 antibody; Tyrosine-protein kinase TYRO5 antibody; Tyrosine-protein kinase receptor EPH-3 antibody; EPHB2 antibody; DRT antibody; EPHT3 antibody; EPTH3 antibody; ERK antibody; HEK5 antibody; TYRO5 antibody
Purified His-tagged EPHB2 protein(Fragment, between amino acids 127~425) was used to produce this monoclonal antibody.
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
WB: 1:1000, FC: 1:10~50, IHC-P: 1:200
Western blot analysis of lysate from EPHB2 protein, using EphB2 Antibody(Cat. #134780). 134780 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35μg.
Flow cytometric analysis of HepG2 cells using EPHB2 Monoclonal Antibody(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EPHB2 Monoclonal Antibody (Cat.#134780), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor.
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