-
Gene name
enhancer of zeste 2 polycomb repressive complex 2 subunit
-
Organism
Human
- Entrez Gene ID
-
Gene Symbol
EZH2
-
Gene Type
protein coding
-
Also Known as
WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
-
Encoded Protein Alias
histone-lysine N-methyltransferase EZH2; enhancer of zeste homolog 2; lysine N-methyltransferase 6
Accession NO.: NM_001203249
-
Product Name
EZH2(NM_001203249) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 5, mRNA.
-
Synonyms
-
ORF length
2088
-
Annotation
This variant (5) has an alternate 5' UTR exon and lacks an in-frame exon and two in-frame segments in the coding region, as compared to variant 1. The resulting isoform (e) lacks three internal segments, as compared to isoform a.
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515887
-
Product Name
EZH2(XM_011515887) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X8, mRNA.
- Synonyms
-
ORF length
2229
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515886
-
Product Name
EZH2(XM_011515886) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X7, mRNA.
-
Synonyms
-
ORF length
2232
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_001203247
-
Product Name
EZH2(NM_001203247) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 3, mRNA.
-
Synonyms
-
ORF length
2241
-
Annotation
This variant (3) lacks an in-frame segment in the coding region, as compared to variant 1. The resulting isoform (c) lacks one internal segment, as compared to isoform a.
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515892
-
Product Name
EZH2(XM_011515892) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X14, mRNA.
-
Synonyms
-
ORF length
2154
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515894
-
Product Name
EZH2(XM_011515894) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X16, mRNA.
-
Synonyms
-
ORF length
2139
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_005249962
-
Product Name
EZH2(XM_005249962) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X3, mRNA.
-
Synonyms
-
ORF length
2265
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515891
-
Product Name
EZH2(XM_011515891) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X13, mRNA.
-
Synonyms
-
ORF length
2157
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515885
-
Product Name
EZH2(XM_011515885) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X5, mRNA.
-
Synonyms
-
ORF length
2253
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515896
-
Product Name
EZH2(XM_011515896) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X21, mRNA.
-
Synonyms
-
ORF length
2022
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515889
-
Product Name
EZH2(XM_011515889) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X11, mRNA.
-
Synonyms
-
ORF length
2190
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515899
-
Product Name
EZH2(XM_011515899) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X25, mRNA.
-
Synonyms
-
ORF length
1536
-
siRNA
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515890
-
Product Name
EZH2(XM_011515890) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X12, mRNA.
-
Synonyms
-
ORF length
2163
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_001203248
-
Product Name
EZH2(NM_001203248) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 4, mRNA.
-
Synonyms
-
ORF length
2214
-
Annotation
This variant (4) lacks two in-frame segments in the coding region, as compared to variant 1. The resulting isoform (d) lacks two internal segments, as compared to isoform a.
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515901
-
Product Name
EZH2(XM_011515901) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X27, mRNA.
-
Synonyms
-
ORF length
1299
-
siRNA
- DataSheet:
- FAQs:
Accession NO.: XM_017011820
-
Product Name
EZH2(XM_017011820) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X19, mRNA.
-
Synonyms
-
ORF length
2112
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_004456
-
Product Name
EZH2(NM_004456) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 1, mRNA.
- Synonyms
-
ORF length
2256
-
Annotation
This variant (1) encodes the longest isoform (a).
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
- DataSheet:
- FAQs:
Accession NO.: XM_011515895
-
Product Name
EZH2(XM_011515895) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X18, mRNA.
-
Synonyms
-
ORF length
2136
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_005249963
-
Product Name
EZH2(XM_005249963) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X6, mRNA.
-
Synonyms
-
ORF length
2238
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_005249964
-
Product Name
EZH2(XM_005249964) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X20, mRNA.
-
Synonyms
-
ORF length
2112
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_152998
-
Product Name
EZH2(NM_152998) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant 2, mRNA.
- Synonyms
-
ORF length
2124
-
Annotation
This variant (2) lacks an in-frame exon and an in-frame segment in the coding region, as compared to variant 1. The resulting isoform (b) lacks two internal segments, as compared to isoform a.
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515893
-
Product Name
EZH2(XM_011515893) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X15, mRNA.
-
Synonyms
-
ORF length
2148
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515883
-
Product Name
EZH2(XM_011515883) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X2, mRNA.
- Synonyms
-
ORF length
2280
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_017011819
-
Product Name
EZH2(XM_017011819) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X17, mRNA.
-
Synonyms
-
ORF length
2139
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_017011818
-
Product Name
EZH2(XM_017011818) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X10, mRNA.
-
Synonyms
-
ORF length
2217
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_011515897
-
Product Name
EZH2(XM_011515897) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X22, mRNA.
- Synonyms
-
ORF length
1929
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_017011821
-
Product Name
EZH2(XM_017011821) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), transcript variant X24, mRNA.
-
Synonyms
-
ORF length
1914
-
siRNA
GCUGACCAUUGGGACAGUAtt
GUGUAUGAGUUUAGAGUCAtt
GGCACUUACUAUGACAAUUtt
- DataSheet:
- FAQs:
-
Vector Name
pCMV-SPORT6
-
primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4396
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
-
-
Protein Tag
None
-
Description
Vector for cloning and transient mammalian cell expression of cDNAs.
-
Vector Name
pEXP-C-EGFP
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6703
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
GFPSpark
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-C-FLAG
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6013
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
FLAG
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-C-HA
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6016
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
HA
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-C-His
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6019
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
His
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-C-Myc
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6019
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
Myc
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-N-EGFP
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6703
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
GFPSpark
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-N-FLAG
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6013
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
FLAG
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-N-mOrange2
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6662
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
OFPSpark
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-untagged
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6223
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Hygromycin
-
Protein Tag
None
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pOTENT-1
-
primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Neomycin
-
Protein Tag
FLAG, 6 His
-
Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
-
Vector Name
pUC18c
-
primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2693
-
Vector Type
Cloning Vector
-
Promoter
lac
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
-
-
Protein Tag
None
-
Description
pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
-
Vector Name
pcDNA3.1-3xFlag
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5508
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Neomycin
-
Protein Tag
Flag
-
Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"