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Gene name
dpy-30 histone methyltransferase complex regulatory subunit
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Organism
Rat
- Entrez Gene ID
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Gene Symbol
Dpy30
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Gene Type
protein coding
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Also Known as
Aip1
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Encoded Protein Alias
dpy-30 homolog; dpy-30-like protein; dpy-30L; protein rAIP1
Accession NO.: NM_173117
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Product Name
Dpy30(NM_173117) Rat cDNA/ORF Clone
- Accession NO.
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Transcript Name
Rattus norvegicus dpy-30 histone methyltransferase complex regulatory subunit (Dpy30), transcript variant 2, mRNA.
- Synonyms
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ORF length
300
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Annotation
This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.
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siRNA
GACUUGCUGUGCUCGCAAAtt
AACAGAAGGUGGAUCUACAtt
AGAGAAUAGUUGAAAAUGAtt
- DataSheet:
- DataSheet:
- FAQs:
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Vector Name
pEXP-Entry
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primers
T7: TAATACGACTCACTATAGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4919
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
Myc,FLAG
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Description
ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
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Vector Name
pGEM-T
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3000
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
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