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Gene name
serine and arginine rich splicing factor 3
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Organism
Cattle
- Entrez Gene ID
-
Gene Symbol
SRSF3
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Gene Type
protein coding
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Also Known as
SFRS3
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Encoded Protein Alias
serine and arginine rich splicing factor 3
Accession NO.: NM_013663
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Product Name
SRSF3(NM_013663) Cattle cDNA/ORF Clone
- Accession NO.
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Transcript Name
Bos taurus serine and arginine rich splicing factor 3 (SRSF3), mRNA.
- Synonyms
-
ORF length
495
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siRNA
GCAGGUCACUUUCUAGAGAtt
GUCGAAUGGUGAAAAGAGAtt
UAGGUAAUCUUGGAAAUAAtt
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- FAQs:
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Vector Name
pCMV-SPORT6
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4396
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Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
-
-
Protein Tag
None
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Description
Vector for cloning and transient mammalian cell expression of cDNAs.
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Vector Name
pEXP-Entry
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primers
T7: TAATACGACTCACTATAGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4919
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Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Neomycin
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Protein Tag
Myc,FLAG
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Description
ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
-
Vector Name
pOTENT-1
-
primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
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Vector Type
Mammalian Expression Vector
-
Promoter
CMV
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Antibiotic Resistance
Kanamycin
-
Selection Marker
Neomycin
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Protein Tag
FLAG, 6 His
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Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Vector Name
pUC19
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primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2686
-
Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
-
Selection Marker
-
-
Protein Tag
None
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Description
pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
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Vector Name
pcDNA3.1-3xFlag
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5494
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Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Neomycin
-
Protein Tag
Flag
-
Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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