-
Gene name
complement C8 beta chain
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Organism
Human
- Entrez Gene ID
-
Gene Symbol
C8B
-
Gene Type
protein coding
-
Also Known as
C82
-
Encoded Protein Alias
complement component 8 subunit beta
Accession NO.: NM_001278543
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Product Name
C8B(NM_001278543) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens complement C8 beta chain (C8B), transcript variant 2, mRNA.
-
Synonyms
-
ORF length
1620
-
Annotation
This variant (2) differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1.
-
siRNA
CUCUCAUACUAAAAGCGUAtt
GACCCAAGGCAAAUACGAAtt
GGUCAAAUGCAGUCAACAAtt
- DataSheet:
- FAQs:
Accession NO.: NM_001278544
-
Product Name
C8B(NM_001278544) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens complement C8 beta chain (C8B), transcript variant 3, mRNA.
-
Synonyms
-
ORF length
1590
-
Annotation
This variant (3) differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (3) with a shorter and distinct N-terminus, compared to isoform 1.
-
siRNA
CUCUCAUACUAAAAGCGUAtt
GACCCAAGGCAAAUACGAAtt
GGUCAAAUGCAGUCAACAAtt
- DataSheet:
- FAQs:
Accession NO.: NM_000066
-
Product Name
C8B(NM_000066) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens complement C8 beta chain (C8B), transcript variant 1, mRNA.
-
Synonyms
-
ORF length
1776
-
Annotation
This variant (1) encodes the longest isoform (1).
-
siRNA
CUCUCAUACUAAAAGCGUAtt
GACCCAAGGCAAAUACGAAtt
GGUCAAAUGCAGUCAACAAtt
- DataSheet:
- DataSheet:
- DataSheet:
- FAQs:
Accession NO.: XM_017002235
-
Product Name
C8B(XM_017002235) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
PREDICTED: Homo sapiens complement component 8, beta polypeptide (C8B), transcript variant X1, mRNA.
-
Synonyms
-
ORF length
1776
-
siRNA
CUCUCAUACUAAAAGCGUAtt
GACCCAAGGCAAAUACGAAtt
GGUCAAAUGCAGUCAACAAtt
- DataSheet:
- FAQs:
-
Vector Name
pCR4-TOPO
-
primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3956
-
Vector Type
Cloning Vector
-
Promoter
lac
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Neomycin
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Protein Tag
None
-
Description
TOPO Cloning vector with ampicillin resistance in bacteria.
-
Vector Name
pGEM-T
-
primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3000
-
Vector Type
Cloning Vector
-
Promoter
lac
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
-
-
Protein Tag
None
-
Description
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
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Vector Name
pOTENT-1
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primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Neomycin
-
Protein Tag
FLAG, 6 His
-
Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Vector Name
pcDNA3.1-3xFlag
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5494
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Neomycin
-
Protein Tag
Flag
-
Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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