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Product Name
c-Myc Polyclonal Antibody
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Description
Polyclonal antibody to c-Myc
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
MYC antibody; MRTL antibody; MYCC antibody; bHLHe39 antibody; c-Myc antibody; myc proto-oncogene protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human c-Myc
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - c-Myc Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using c-Myc antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"