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  • BCKDK Antibody (C-term T340)
  • BCKDK Antibody (C-term T340)

BCKDK Antibody (C-term T340)

Cat.#: 134778

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Special Price 372.6 USD

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Product Information

  • Product Name
    BCKDK Antibody (C-term T340)
  • Documents
  • Description
    Purified Rabbit Polyclonal Antibody (Pab)
  • Tested applications
    WB, ELISA
  • Species reactivity
    Human, Mouse
  • Alternative names
    [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody; mitochondrial antibody; Branched-chain alpha-ketoacid dehydrogenase kinase antibody; BCKD-kinase antibody; BCKDHKIN antibody; BCKDK antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This BCKDK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325-356 amino acids from the C-terminal region of human BCKDK.
  • Clonality
    Polyclonal
  • Formulation
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Storage instructions
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
  • Applications
    WB: 1:1000
  • Validations

    The anti-BCKDK Pab (Cat. #134778) is used in Western blot to detect BCKDK in mouse intestine tissue lysate (Lane 1) and Hela cell lysate (Lane 2).

    The anti-BCKDK Pab (Cat. #134778) is used in Western blot to detect BCKDK in mouse intestine tissue lysate (Lane 1) and Hela cell lysate (Lane 2).

    Western blot analysis of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody (C-term T340) (RB05257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies).

    Western blot analysis of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody (C-term T340) (RB05257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies).

  • Background
    Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"