AVPR2 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
WB, FC, ELISA
ADHR antibody; DIR antibody; DIR3 antibody; V2R antibody; Vasopressin V2 receptor antibody; V2R antibody; AVPR V2 antibody; Antidiuretic hormone receptor antibody; Renal-type arginine vasopressin receptor antibody; AVPR2 antibody; ADHR antibody; DIR antibody; DIR3 antibody; V2R antibody
This AVPR2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 343-377 amino acids from the C-terminal region of human AVPR2.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
FC: 1:25, WB: 1:2000
Overlay histogram showing Jurkat cells stained with 139381 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( , 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-AVPR2 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.
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