ATP6V1A Polyclonal Antibody
Polyclonal antibody to ATP6V1A
Human, Mouse, Rat
ATP6V1A antibody; ATP6A1 antibody; ATP6V1A1 antibody; HO68 antibody; VA68 antibody; VPP2 antibody; Vma1 antibody; ARCL2D antibody; ATPase H+ transporting V1 subunit A antibody
Antigen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ATP6V1A (NP_001681.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:100 - 1:200
Western blot - ATP6V1A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATP6V1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.
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