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Product Name
ATP6V1A Polyclonal Antibody
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Description
Polyclonal antibody to ATP6V1A
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ATP6V1A antibody; ATP6A1 antibody; ATP6V1A1 antibody; HO68 antibody; VA68 antibody; VPP2 antibody; Vma1 antibody; ARCL2D antibody; ATPase H+ transporting V1 subunit A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ATP6V1A (NP_001681.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - ATP6V1A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATP6V1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
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Background
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.
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References
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"