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Gene name
alpha-2-macroglobulin
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Organism
Human
- Entrez Gene ID
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Gene Symbol
A2M
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Gene Type
protein coding
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Also Known as
A2MD; CPAMD5; FWP007; S863-7
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Encoded Protein Alias
alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; alpha-2-M
Accession NO.: XM_006719056
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Product Name
A2M(XM_006719056) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
PREDICTED: Homo sapiens alpha-2-macroglobulin (A2M), transcript variant X1, mRNA.
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Synonyms
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ORF length
4539
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siRNA
CAGUGAUGGUUAAGAACGAtt
CGGAGACCGUACGAAAGUAtt
CAAUCGUCAUAAUGUCUAUtt
- DataSheet:
- FAQs:
Accession NO.: NM_000014
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Product Name
A2M(NM_000014) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
Homo sapiens alpha-2-macroglobulin (A2M), mRNA.
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Synonyms
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ORF length
4425
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siRNA
CAGUGAUGGUUAAGAACGAtt
CGGAGACCGUACGAAAGUAtt
CAAUCGUCAUAAUGUCUAUtt
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- FAQs:
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Vector Name
pCMV-SPORT6
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4396
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
Vector for cloning and transient mammalian cell expression of cDNAs.
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Vector Name
pOTENT-1
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primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
FLAG, 6 His
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Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Vector Name
pUC18c
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primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2693
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
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Vector Name
pcDNA3.1-3xFlag
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5494
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
Neomycin
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Protein Tag
Flag
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Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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