ADM Antibody (Center)
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
FC, WB, ELISA
AM antibody; ADM antibody; Adrenomedullin antibody; AM antibody; Proadrenomedullin N-20 terminal peptide antibody; ProAM N-terminal 20 peptide antibody; PAMP antibody; ProAM-N20 antibody; ADM antibody; AM antibody
This ADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-96 amino acids from the Central region of human ADM.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
WB: 1:1000, FC: 1:10~50
Anti-ADM Antibody (Center) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing A549 cells stained with 134847 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (134847, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-ADM Antibody (Center) at 1:1000-1:2000 dilution Lane 1: human breast lysate Lane 2: human kidney lysate Lane 3: human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of ADM Antibody (Center) (Cat. #134847) in mouse lung tissue lysates (35ug/lane).ADM (arrow) was detected using the purified Pab.
ADM Antibody (Center) (Cat. #134847) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.
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