NT5C1A Human cDNA/ORF Clone

pEXP-untagged

T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG

6223

Mammalian Expression Vector

CMV

Ampicillin

Hygromycin

None

All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.

pOTENT-1

pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC

7510

Mammalian Expression Vector

CMV

Kanamycin

Neomycin

FLAG, 6 His

ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.

pUC18c

M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC

2693

Cloning Vector

lac

Ampicillin

-

None

pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.