NovoMonoTM, a Freeland for any desired antibodies
Leading technologies
NovoFocusTM--focus on the unique sequences, unstructured and exposed regions.
Precise antigens design is vital for successful antibody production. To reach better antibody-antigen recognition of most applications, NovoFocusTM, developed by NovoPro's bioinformatics team, is able to predict, evaluate protein structure information and effectively select the unstructured or loop regions of proteins. To guarantee the specificity, a novel sequence comparison algorithm is developed which can screen the possible peptide sequences in proteome level and calculate similarities in an antibody development view. More than 20 factors have been integrated to the comprehensive NovoFocusTM antigen design, lead to a precise value of epitopes and customer-friendly interface.
The major parameters consist of NovoFocusTM epitope value
| Structure Information | Protein Regions |
| α-helix/ β sheet/ loop | Signal peptide (avoided) |
| Amino Acid Characteristics | Transmembrane region (avoided) |
| Antigenic value of amino acid | Possibly modified regions |
| Flexibility | Disordered regions |
| Sequence specificity | Solvent accessibility |
| Blast target species and rabbit | N-terminal, C-terminal |
| Requirements of Customers | Evolution |
| Specified regions | Positive selection |
| Recognize multiple proteins | Discrimination |
| Differentiate specific proteins |
NovoBoostTM—best adjuvant leads to highest affinity
Affinity is key for antibody effectiveness, especially important for low abundance proteins and high requirement applications. To greatly improve the antibody, a novel immunogenicity-amplified adjuvant - NovoBoostTM is developed. NovoBoostTM is composed from small (<10kD) dendrimers positively charged at their surface. When the antigen is mixed with NovoBoostTM, the large, positively charged complex molecule is obtained, which effectively binds to antigen receptors on dendritic cells, and effectively stimulate T-helper activity, thus inducing strong and specific immune response. Thanks to the tremendously enhanced immunogenicity, 90% of the NovoPro antibodies could reach 1ng-20ng detection limit (Fig 1.1 & Fig 1.2).
Helpful tech support, experiencing true partnership
Your success is our mission.
NovoPro scientific team has years of antibody validation and application experiences. Not only you can find various of antibody information such as antibody user guide, FAQs, tips, but also you are always welcome to discuss with our experts about any thoughts and problems in experiments. We would try our best to make your research easy and enjoyable.
Product Selection
Monoclonal antibody production using protein as antigen
Protein antigens are the elite choice when it comes to high-quality monoclonal antibody production. Their unique ability to elicit monoclonal antibodies that recognize the native confirmation of the target protein makes them a superior choice over other antigen strategies.
| Package | NovoMono Protein |
| Guarantees | WB positive for immunogen; ELISA ≥ 1:64,000 |
| Starting Material | Target protein name, sequence or accession number NovoPro will produce the protein antigen |
| Immunization | 5 Balb/c mice |
| Customer Screening | 5 ELISA(+) supernatants for evaluation in client's own experimental system |
| Deliverables | 1mg recombinant protein; 2-5 hybridoma cell lines of customer's choice+; 2-5 mg purified mAb from two chosen hybridoma |
| Final Delivery | 21-26 weeks |
| Cost | $2,998 |
*additional cell lines and purified antibody can be provided as $399/clone and $399/2mg, clone respectively
Monoclonal antibody production using peptide as antigen
In cases where access to a target protein is limited or unavailable, but the amino acid sequence is known, peptide antigens offer a smart solution for monoclonal antibody production. Peptide antigens are also ideal for producing monoclonal antibodies against post-translational modification sites within a target protein.
| Package | Starting Material | Antigen | Immunization | Customer Screening | First Delivery | Cost |
|---|---|---|---|---|---|---|
| NovoMonoTM Protein Step 1 | Target protein name, sequence or accession number. | NovoPro will design and produce the 4 peptide antigens | 4 Balb/c mice | 4 ELISA(+) sera in client's own experimental system | 10-12 weeks | $999 |
The customer chooses the best 2 animals from among the original 5 mice. These best-responding animals are boosted twice more and then their spleen cells harvested for hybridoma fusion (10 plates per fusion). An ELISA assay is used to screen all positive supernatants, and in the end 2-5 best cell lines will be chosen and proceed to subcloning and final hybridoma.
| Package | Starting | Deliverables | Second Delivery | Cost |
|---|---|---|---|---|
| NovoMonoTM Protein Step 2 | 2 Chosen mice | 2-5 hybridoma clones* Pre-immune sera as controls 2-5mg purified antibodies from two cell lines. 2-3mg Purified peptides Project report |
10-12 weeks | $1998 |
*additional cell lines and purified antibody can be provided as $399/clone and $399/2mg, clone respectively
Timelines
| STEP | Estimated Time | Description |
|---|---|---|
STEP1: |
||
Preimmune |
Day 0 |
|
1st Immunization |
Day 1 |
|
2nd Immunization |
Day 14 |
|
3rd Immunization |
Day 21 |
|
Test Bleed & ELISA Test |
Day 28 |
|
4th Immunization |
Day 39 |
|
5th Immunization |
Day 60 |
Optional |
6th Immunization |
Day 68 |
Optional |
STEP2 & STEP3: |
||
Spleen cell Isolation & Fusion |
Day 43 or Day 70 |
Depending on the titer we can perform additional immunizations before fusion |
STEP4: |
||
ELISA test and QC |
Day 53 or Day 80 |
|
STEP5: |
||
Subcloning, Production and purification |
Day 83 or Day 110 |
|
Most thoughtful service procedure
Project inquiry |
Tel: +86-021-61842887 |
Project initial plan |
Contact our experienced antibody consultant and form a brief antibody development plan together. |
Service agreement |
Customer confirmation of the proposal, a service agreement signed by both parties. If possible, a Purchase Order (PO) is desired to be issued by the financial office. |
Project initiation |
Project(s) will be initiated immediately on the receive of the agreement or PO. |
Project status |
Customer will receive an account in Customer Center to track the project status, the status will be updated once a week. |
Sending QC reports |
The antibody QC reports will be sent to customer when the project completes, and a payment or PO is expected to be made by the successful completion of the project. |
Delivery |
Delivery through DHL, domestic: 2-3 working days, oversea: 3-5 working days. |
Tech support |
Any question about antibody validation or suggestions, please contact: support@novoprolabs.com |