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pAAV2-7M8 vector (V012820)

Basic Vector Information

AAV packaging plasmid, expressing Rep2 and 7M8 capsid. 7mer insertion in the AAV2 cap

Vector Name:
pAAV2-7M8
Antibiotic Resistance:
Ampicillin
Length:
8369 bp
Type:
Viral Expression & Packaging Vectors
Replication origin:
ori
Growth Strain(s):
NEB Stable
Growth Temperature:
37℃

pAAV2-7M8 vector Vector Map

pAAV2-7M88369 bp400800120016002000240028003200360040004400480052005600600064006800720076008000ITRRep78WELQut siteM13 oriAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pAAV2-7M8 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_3240        8369 bp DNA     circular SYN 21-AUG-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   8..110
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     CDS             258..2114
                     /gene="Rep78"
                     /label=Rep78
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             4213..4224
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
     rep_origin      5150..5663
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6410..6514
                     /label=AmpR promoter
     CDS             6515..7372
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7546..8134
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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