Basic Vector Information
AAV packaging plasmid, expressing Rep2, and rh10 capsid
- Vector Name:
- pAAV2/rh10
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7336 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Promoter:
- Truncated P5
- Growth Strain(s):
- DH5alpha
- Growth Temperature:
- 37℃
pAAV2/rh10 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pAAV2/rh10 vector Sequence
LOCUS 40924_3252 7336 bp DNA circular SYN 21-AUG-2023 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7336) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7336 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 146..162 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 172..190 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 220..236 /label=KS primer /note="common sequencing primer, one of multiple similar variants" CDS 291..2153 /gene="Rep78" /label=Rep78 /note="Protein Rep78 from Adeno-associated virus 2 (isolate Srivastava/1982). Accession#: Q89268" CDS 4231..4242 /label=WELQut site /note="WELQut protease recognition and cleavage site" promoter complement(4694..4712) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4733..4749) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4757..4773) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4781..4811) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4826..4847) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5135..5723) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5897..6754) /label=AmpR /note="beta-lactamase" promoter complement(6755..6859) /label=AmpR promoter rep_origin complement(join(6885..7336,1..4)) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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