Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V012790 | pRSV-Rev | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE and envelope expressing plasmid.
- Vector Name:
- pRSV-Rev
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4174 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- Didier Trono
- Copy Number:
- High copy number
- Promoter:
- RSV
- Growth Strain(s):
- JM108
- Growth Temperature:
- 37℃
pRSV-Rev vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Farzaneh M, Sayyah M, Eshraghi HR, Panahi N, Mirzapourdelavar H, Gholami Pourbadie H. The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus. Iran Biomed J. 2019 Sep;23(5):324-9. doi: 10.29252/.23.5.324. Epub 2019 May 19. PMID: 31103020; PMCID: PMC6661131.
pRSV-Rev vector Sequence
LOCUS 40924_38283 4174 bp DNA circular SYN 13-DEC-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4174) TITLE Direct Submission REFERENCE 2 (bases 1 to 4174) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4174 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 107..128 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 365..627 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" CDS 756..1103 /codon_start=1 /label=Rev /note="human immunodeficiency virus 1 (HIV-1) Rev protein" /translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRW RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV ESPTILESGAKE" primer_bind complement(1190..1206) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 1532..1987 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2269..2373 /label=AmpR promoter CDS 2374..3231 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3405..3993 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"