Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V000011 | pAdDeltaF6 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pAdDeltaF6
- Antibiotic Resistance:
- Ampicillin
- Length:
- 15420 bp
- Type:
- AAV
- Replication origin:
- ori
- Copy Number:
- Low Copy
- Promoter:
- tet
pAdDeltaF6 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pAdDeltaF6 vector Sequence
LOCUS pAdDeltaF6. 15420 bp DNA circular SYN 19-OCT-2021
DEFINITION AAV helper plasmid.
ACCESSION .
VERSION .
KEYWORDS pAdDeltaF6.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15420)
TITLE AAV packaging plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 15420)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 15420)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..15420
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(142..158)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(195..213)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(234..250)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(258..274)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(282..312)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(327..348)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(465..482)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(636..1224)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1398..2255)
/label=AmpR
/note="beta-lactamase"
promoter complement(2256..2360)
/label=AmpR promoter
primer_bind 2428..2446
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 2473..2501
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
repeat_region 3013..3115
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
CDS 3425..3808
/note="Early 4 ORF1 protein from Human adenovirus C
serotype 2. Accession#: P03242"
CDS 4248..4595
/note="Early 4 ORF3 protein from Human adenovirus C
serotype 2. Accession#: P03241"
CDS 4874..5755
/note="Early 4 ORF6 protein from Human adenovirus C
serotype 2. Accession#: P03239"
CDS complement(6167..7909)
/note="Fiber protein from Human adenovirus C serotype 5.
Accession#: P11818"
CDS complement(8444..9124)
/gene="L4"
/label=L4
/note="Pre-hexon-linking protein VIII from Human adenovirus
C serotype 5. Accession#: P24936"
CDS complement(9811..12231)
/gene="L4"
/label=L4
/note="Shutoff protein from Human adenovirus C serotype 5.
Accession#: P24933"
CDS 12260..13846
/gene="DBP"
/label=DBP
/note="DNA-binding protein from Human adenovirus C serotype
5. Accession#: P03265"