Basic Vector Information
- Vector Name:
- pAAV2/2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7468 bp
- Type:
- Mammalian Expression, AAV
- Replication origin:
- ori
- Copy Number:
- High Copy
- Promoter:
- p5
- 5' Primer:
- M13 Reverse
pAAV2/2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pAAV2/2 vector Sequence
LOCUS pAAV2-2. 7468 bp DNA circular SYN 19-DEC-2023
DEFINITION AAV packaging plasmid expressing Rep/Cap genes for production of
serotype 2.
ACCESSION .
VERSION .
KEYWORDS pAAV2-2.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7468)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 7468)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7468
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 132..151
/label=pBR322ori-F
/note="pBR322ori-F"
/note="pBR322 origin, forward primer"
primer_bind 385..402
/label=L4440
/note="L4440"
/note="L4440 vector, forward primer"
protein_bind 519..540
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 555..585
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 593..609
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 617..633
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 683..699
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 713..760
/label=FRT
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 919..2781
/gene="Rep78"
/label=Rep78
/note="Protein Rep78 from Adeno-associated virus 2 (isolate
Srivastava/1982). Accession#: Q89268"
CDS 2801..5005
/gene="VP1"
/label=VP1
/note="Capsid protein VP1 from Adeno-associated virus 2
(isolate Srivastava/1982). Accession#: P03135"
primer_bind complement(5336..5352)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(5493..5948)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5975..6079
/label=AmpR promoter
CDS 6080..6937
/label=AmpR
/note="beta-lactamase"
rep_origin 7111..7468
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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