Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V001257 | pAAV2/9n | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pAAV2/9n is a AAV packaging plasmid expressing Rep/Cap genes.This is a vector developed by Dr. James M. Wilson.
- Vector Name:
- pAAV2/9n
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7330 bp
- Type:
- AAV
- Replication origin:
- ori
- Promoter:
- Truncated P5
- 5' Primer:
- KS primer
- Growth Strain(s):
- stbl3
- Growth Temperature:
- 37℃
pAAV2/9n vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Hu Y, Liu L, Chen Y, et al. Cancer-cell-secreted miR-204-5p induces leptin signalling pathway in white adipose tissue to promote cancer-associated cachexia. Nat Commun. 2023;14(1):5179. Published 2023 Aug 24. doi:10.1038/s41467-023-40571-9
pAAV2/9n vector Sequence
LOCUS pAAV2-9n. 7330 bp DNA circular SYN 19-DEC-2023
DEFINITION AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION .
VERSION .
KEYWORDS pAAV2-9n.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7330)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 7330)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7330
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 137..153
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 163..181
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 211..227
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 282..2144
/gene="Rep78"
/label=Rep78
/note="Protein Rep78 from Adeno-associated virus 2 (isolate
Srivastava/1982). Accession#: Q89268"
CDS 4216..4227
/label=WELQut site
/note="WELQut protease recognition and cleavage site"
promoter complement(4679..4697)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4718..4734)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4742..4758)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4766..4796)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4811..4832)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4949..4966)
/label=L4440
/note="L4440"
/note="L4440 vector, forward primer"
rep_origin complement(5120..5708)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5882..6739)
/label=AmpR
/note="beta-lactamase"
promoter complement(6740..6844)
/label=AmpR promoter
rep_origin complement(6870..7325)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"