Basic Vector Information
- Vector Name:
- pCDNA3.1 myc-NL-GW
- Antibiotic Resistance:
- Ampicillin, Chloramphenicol
- Length:
- 7607 bp
- Type:
- Signal transduction
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- SV40
- Growth Strain(s):
- DB3.1
- Growth Temperature:
- 37℃
pCDNA3.1 myc-NL-GW vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCDNA3.1 myc-NL-GW vector Sequence
LOCUS 62056_6000 7607 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7607) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7607 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 103..482 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 483..686 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 731..749 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 775..804 /codon_start=1 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" /translation="EQKLISEEDL" CDS 802..1314 /codon_start=1 /label=Nluc /note="NanoLuc(R) luciferase" /translation="LVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW RLCERILA" protein_bind 1321..1445 /label=attR1 /note="recombination site for the Gateway(R) LR reaction" promoter 1470..1500 /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" CDS 1554..2231 /codon_start=1 /label=CmR /note="chloramphenicol acetyltransferase" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE DPAY" CDS 2554..2856 /codon_start=1 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" protein_bind complement(2900..3024) /label=attR2 /note="recombination site for the Gateway(R) LR reaction" polyA_signal 3072..3296 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3342..3770 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3784..4113 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 4180..4971 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 5148..5281 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5318..5334) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(5342..5358) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5366..5396) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(5411..5432) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5720..6308) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6482..7339) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7340..7444) /label=AmpR promoter
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