Basic Vector Information
- Vector Name:
- pFBDM
- Antibiotic Resistance:
- Ampicillin, Gentamycin
- Length:
- 5279 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Insect cells
- Selection Marker:
- Gen
- Promoter:
- p10
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pFBDM vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pFBDM vector Sequence
LOCUS 62056_10810 5279 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5279) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5279 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 106..560 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 586..690 /label=AmpR promoter CDS 691..1548 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1722..2310 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" mobile_element complement(2615..2839) /label=Tn7R /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2909..3439) /codon_start=1 /label=GmR /note="gentamycin acetyltransferase" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3628..3656) /label=Pc promoter /note="class 1 integron promoter" polyA_signal complement(4185..4233) /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" promoter complement(4369..4478) /label=p10 promoter /note="baculovirus promoter for expression in insect cells" promoter 4523..4614 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" polyA_signal 4873..5007 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" mobile_element complement(5036..5201) /label=Tn7L /note="mini-Tn7 element (left end of the Tn7 transposon)"
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