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Product Name
USP13 antibody
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Description
USP13 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HEK-293 cells, A375 cells, HeLa cells, mouse testis tissue, rat testis tissue. Positive IHC detected in human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, mouse heart tissue. Observed molecular weight by Western-blot: 97-100 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Deubiquitinating enzyme 13 antibody; Isopeptidase T 3 antibody; IsoT 3 antibody; ISOT3 antibody; Ubiquitin thiolesterase 13 antibody; USP13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP13 recombinant protein (Accession Number: NM_003940). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116590(USP13 antibody) at dilution of 1:400
IP Result of anti-USP13 (IP:Catalog No:116590, 5ug; Detection:Catalog No:116590 1:500) with HeLa cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human testis using Catalog No:116590(USP13 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:116590(USP13 antibody) at dilution of 1:50 (under 40x lens)
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Background
USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.
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References
- Vakifahmetoglu-Norberg H, Kim M, Xia HG. Chaperone-mediated autophagy degrades mutant p53. Genes & development. 27(15):1718-30. 2013.
- Yoshioka Y, Ye YQ, Okada K. Ubiquitin-specific peptidase 5, a target molecule of vialinin A, is a key molecule of TNF-α production in RBL-2H3 cells. PloS one. 8(12):e80931. 2013.
- Xia HG, Najafov A, Geng J. Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death. The Journal of cell biology. 210(5):705-16. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"