Anti-TNFRSF9 antibody

Cat.#: 176678

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Product Information

  • Product Name
    Anti-TNFRSF9 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to TNFRSF9
  • Tested applications
    WB, IHC-P, ICC, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ILA antibody; 4-1BB antibody; CD137 antibody; CDw137 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500

    ICC: 1:100-1:500

    IHC-P: 1:50-1:200

    FC: 1:50-1:200

  • Validations

    Fig1: Western blot analysis of CD137 on HepG2 cell lysate using anti-CD137 antibody at 1/500 dilution.

    Fig1: Western blot analysis of CD137 on HepG2 cell lysate using anti-CD137 antibody at 1/500 dilution.

    Fig2: ICC staining CD137 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining CD137 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining CD137 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining CD137 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining CD137 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining CD137 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.

    Fig10: Flow cytometric analysis of Jurkat cells with CD137 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

    Fig10: Flow cytometric analysis of Jurkat cells with CD137 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

  • Background
  • References
    • Kang SW et al. Anti-CD137 suppresses tumor growth by blocking reverse signaling by CD137 ligand. Cancer Res. pii: canres (2017).
    • Hebb JPO et al. Administration of low-dose combination anti-CTLA4, anti-CD137, and anti-OX40 into murine tumor or proximal to the tumor draining lymph node induces systemic tumor regression.Cancer Immunol Immunother.(2017).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"