Anti-Gmnn antibody

Cat.#: 176698

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Product Information

  • Product Name
    Anti-Gmnn antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Gmnn
  • Tested applications
    ICC, IHC-P, FC
  • Species reactivity
    Mouse
  • Alternative names
    Gem antibody; AW546347 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was recombinant protein with mouse geminin aa 70-206 / 206.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    IHC-P: 1:100-1:200

    ICC: 1:200

  • Validations

    Fig1: ICC staining Geminin in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

    Fig1: ICC staining Geminin in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

    Fig2: ICC staining Geminin in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

    Fig2: ICC staining Geminin in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

    Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- Geminin rabbit polyclonal antibody.

    Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- Geminin rabbit polyclonal antibody.

  • Background
  • References
    • Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity. Yanagi K., Mizuno T., You Z., Hanaoka F. J. Biol. Chem. 277:40871-40880(2002)
    • Structural basis for inhibition of the replication licensing factor Cdt1 by geminin. Lee C., Hong B., Choi J.M., Kim Y., Watanabe S., Ishimi Y., Enomoto T., Tada S., Kim Y., Cho Y. Nature 430:913-917(2004)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"