Anti-AGO3 antibody

Cat.#: 176538

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Product Information

  • Product Name
    Anti-AGO3 antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to AGO3
  • Tested applications
    WB, ICC/IF, IHC-P, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    EIF2C3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000

    ICC/IF: 1:100-1:500

    IHC-P: 1:50-1:200

    FC: 1:50-1:100

  • Validations

    Fig1: ICC staining EIF2C3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig1: ICC staining EIF2C3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining EIF2C3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining EIF2C3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining EIF2C3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining EIF2C3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining EIF2C3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining EIF2C3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

    Fig9: Flow cytometric analysis of N2A cells with EIF2C3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary

    Fig9: Flow cytometric analysis of N2A cells with EIF2C3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary

  • Background
  • References
    • Hein M.Y., et al. 2015. A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 163:712-723.
    • Schurmann N., et al. 2013. Molecular dissection of human Argonaute proteins by DNA shuffling. Nat. Struct. Mol. Biol. 20:818-826.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"